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Peptides

What Is Kisspeptin-10? A Research Overview

Explicit Research  ·  Research Compound Overview
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Research Use Only. Kisspeptin-10 is sold by Explicit Research exclusively for laboratory and research purposes. This article summarizes published preclinical and experimental literature. It is not medical advice and does not constitute a recommendation for human use. All compounds are for in vitro and animal research only.

All information below describes the compound's chemical identity, laboratory handling, and the published research literature. It describes molecular targets and results in laboratory and animal models only — not effects in humans — and is not evidence of any human benefit.

What Is Kisspeptin-10?

Kisspeptin-10 (also written Kp-10; historically termed metastin 45–54) is a synthetic decapeptide composed of 10 amino acids, sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2 (YNWNSFGLRF-NH2). It corresponds to the shortest C-terminal fragment of the kisspeptin family of peptides encoded by the KISS1 gene, and it retains binding to the KISS1R receptor (also designated GPR54). The KISS1 gene was first described in the mid-1990s, and kisspeptins were characterized as the endogenous ligands of KISS1R/GPR54 in 2001. Like other members of the RFamide peptide family, Kisspeptin-10 carries a C-terminal amide (–Arg-Phe-NH2).

It is supplied as a reference compound for in vitro and animal research use only. The sections below summarize its chemical identity, laboratory handling, the molecular targets and model systems examined in the published literature, and the primary references — without describing outcomes or effects in humans.

Research Targets & Pathways

Published preclinical literature has examined Kisspeptin-10 in relation to several molecular systems. These are receptor and pathway associations reported in laboratory and animal models; refer to the cited studies for methods and findings.

Model Systems Studied

Kisspeptin-10 has been used as a test compound across a range of published preclinical model systems, primarily in rodents and in vitro cell assays. Refer to the cited literature for study designs, endpoints, and findings.

Note: several kisspeptin studies have appeared only recently in the literature; independent replication of the most recent findings remains ongoing.

Stability & Handling

Kisspeptin-10 is a C-terminally amidated linear decapeptide, a structural feature shared across the RFamide peptide family. It is handled as a lyophilized solid and reconstituted in aqueous buffer for laboratory use. Published methodological reports note that, like many short peptides, it is subject to enzymatic degradation in biological matrices and to adsorption onto container and labware surfaces at low concentrations; carrier proteins such as 0.1% bovine serum albumin (BSA) are commonly added to dilute working solutions to limit surface adsorption. These are handling considerations for in vitro and animal research and are not statements about activity in humans.

Molecular & Technical Profile

C63H83N17O14  |  MW ~1302.45 g/mol  |  CAS 374675-21-5  |  Sequence: YNWNSFGLRF-NH2

Storage, Reconstitution & Working Concentrations

Lyophilized storage−20°C, desiccated, protected from light and moisture; reported stable ~24 months
Reconstituted storage2–8°C up to ~28 days; aliquot and freeze at −20°C or −80°C for long-term; limit freeze–thaw cycles
SolubilitySoluble in sterile water and aqueous buffers (e.g. PBS, pH 7.4); 0.1% BSA/PBS to reduce adsorption; DMSO not recommended as primary solvent
ReconstitutionBring vial to room temperature; add solvent slowly down the vial wall — not directly onto the cake; swirl gently — do not vortex
Stock solution0.5–1.0 mg/mL in sterile water or PBS (pH 7.4); add 0.1% BSA for dilute working solutions
In vitro working range1 nM–1 µM; commonly 10–100 nM in KISS1R activation and GnRH/gonadotropin secretion assays (GT1-7, LβT2 lines); titrate per cell system
Animal model dosingSee cited literature for model-specific concentration ranges and administration protocols
Endotoxin<1 EU/mg (LAL assay); confirm lot-specific COA before use in sensitive cell systems

Storage, reconstitution, and working-concentration values are general laboratory guidance for in vitro and animal research; always confirm against the lot-specific Certificate of Analysis.

Current Research Status

As of the time of this writing, Kisspeptin-10 has not been approved by the U.S. Food and Drug Administration (FDA) for any human clinical use. The available evidence base is drawn largely from preclinical systems — cell lines, rodent models, and other animal models — together with investigational clinical research conducted under institutional oversight. Separate, approved drug products act elsewhere on the hypothalamic–pituitary–gonadal axis: for example, triptorelin (marketed as Trelstar) is an approved gonadotropin-releasing hormone (GnRH) agonist, and sermorelin is a separate approved agent. Those are distinct, approved drug products; the Kisspeptin-10 described here is a research-grade material supplied for laboratory use only and is not any approved drug. Ongoing research continues to characterize the compound's receptor pharmacology and model-system profile.

Research FAQ

Is Kisspeptin-10 approved for human use?

No. Kisspeptin-10 has not been approved by the FDA for any human clinical use. The evidence base is drawn from preclinical systems (cell-line, rodent, and other animal models) together with investigational clinical research conducted under institutional oversight, and the compound is supplied for laboratory research use only — not for human consumption.

What is Kisspeptin-10's molecular formula and sequence?

A synthetic decapeptide, sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2 (YNWNSFGLRF-NH2) — molecular formula C63H83N17O14, MW approximately 1302.45 g/mol, CAS 374675-21-5.

How is Kisspeptin-10 stored and reconstituted?

Store lyophilized at −20°C, desiccated and protected from light. Reconstitute in sterile water or aqueous buffer (e.g. PBS, pH 7.4); store the reconstituted solution at 2–8°C for up to ~28 days, aliquot and freeze for longer-term storage, and limit freeze–thaw cycles.

What targets has Kisspeptin-10 been studied for?

Preclinical work has characterized Kisspeptin-10 as a ligand of the KISS1R (GPR54) receptor and examined downstream Gq/11–PLC–calcium and ERK1/2 signaling, the hypothalamic GnRH axis, and arcuate KNDy neurons across cell-line and rodent model systems. Note the recent-literature caveat above.

Selected References

  1. Kotani M, et al. (2001). The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. Journal of Biological Chemistry, 276(37):34631–36.
  2. Ohtaki T, et al. (2001). Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature, 411(6837):613–17.
  3. de Roux N, et al. (2003). Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54. Proceedings of the National Academy of Sciences, 100(19):10972–76.
  4. Seminara SB, et al. (2003). The GPR54 gene as a regulator of puberty. New England Journal of Medicine, 349(17):1614–27.
  5. Messager S, et al. (2005). Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54. Proceedings of the National Academy of Sciences, 102(5):1761–66.
  6. Gottsch ML, et al. (2004). A role for kisspeptins in the regulation of gonadotropin secretion in the mouse. Endocrinology, 145(9):4073–77.
  7. Navarro VM, et al. (2009). Regulation of gonadotropin-releasing hormone secretion by kisspeptin/dynorphin/neurokinin B neurons in the arcuate nucleus of the mouse. Journal of Neuroscience, 29(38):11859–66.